Silver Electrode as a Sensor for Determination of Zinc in Cell Cultivation Medium
| Authors | |
|---|---|
| Year of publication | 2002 |
| Type | Article in Periodical |
| Magazine / Source | Analytical Biochemistry |
| MU Faculty or unit | |
| Citation | |
| Field | Electrochemistry |
| Keywords | silver electrode; cyclic voltammetry; zinc in growth medium; inducible gene expression; BM2 cells; immunoblotting |
| Description | Use of the silver electrode as a sensor for monitoring of zinc in cell growth medium is described. Zinc at siver electrodes provides specific voltammetric signal, which is affected by solution components. Signlas of zinc ions in phosphate buffer solutions with and without cell growth meidum were compared. Common DMEM cell culture medium was used for the cultivation of a cell line of v-myb-transformed chicken monoblasts and its variants expressing v-jun and c-jun in a zinc dependent manner. Electrochemical results showed zinc concentrations in the medium coincide very well with the jun expression. With respect to the low toxicity of xilver for eukaryotic cells, silver electrodes represent promising tools for the determination of zinc concentrations in vivo without the potential risk of a cell culture damage. |
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