Využití preparativní isoelektrické fokusace s imobilizovaným gradientem při purifikaci proteinů
| Title in English | The use of preparative isoelectric focusation with immobilized gradient for protein purification |
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| Authors | |
| Year of publication | 2002 |
| Type | Article in Periodical |
| Magazine / Source | Chemické listy |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | protein purification; isoelectric focusation; Acidithiobacillus |
| Description | A multichamber electrofocusing unit PI 8 IsoPrime using membranes with immobilized pH was applied. Proteins of an egg albumen were used as a model system. pH values were 4.5, 5.5, 8,0 and 9.5. Separation ability was tested using electroforesis. The apparatus was also used for soluble and membrane immobilized cytochrome separation from Acidithiobacillus ferrooxidans. |
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