Využití preparativní isoelektrické fokusace s imobilizovaným gradientem při purifikaci proteinů

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Title in English The use of preparative isoelectric focusation with immobilized gradient for protein purification
Authors

DRÁBKOVÁ Lenka JANICZEK Oldřich MANDL Martin

Year of publication 2002
Type Article in Periodical
Magazine / Source Chemické listy
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords protein purification; isoelectric focusation; Acidithiobacillus
Description A multichamber electrofocusing unit PI 8 IsoPrime using membranes with immobilized pH was applied. Proteins of an egg albumen were used as a model system. pH values were 4.5, 5.5, 8,0 and 9.5. Separation ability was tested using electroforesis. The apparatus was also used for soluble and membrane immobilized cytochrome separation from Acidithiobacillus ferrooxidans.
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