Ergosterol induces mobilization of internal calcium in tobacco cells

• Authors: KAŠPAROVSKÝ Tomáš; MILAT ML; BLEIN JP; MIKEŠ Vladimír
• Year of publication: 2002
• Type: Article in Proceedings
• Conference: Proc. 6th Conf. EFPP, Prague. Plant Protect. Sci
• MU Faculty or unit: Faculty of Science
• Field: Biochemistry
• Keywords: ergposterol; plant protection; tobacco; elicitation
• Description: Ergosterol interacts with tobacco suspension cells and triggers pH changes of extracellular medium, oxidative burst and synthesis of phytoalexins. Cryptogein stimulated an apparent continuous uptake of external calcium within 40 min, whereas no net uptake of external calcium occurred upon the addition of ergosterol. However, the elicitation with both cryptogein and ergosterol resulted in an increase of the fluorescence of calcium green 1 in cytosol. The use of several inhibitors of calcium channels (La3+, TMB-8, verapamil, ruthenium red, nifedipine) and a protein-kinase inhibitor (staurosporin) suggests that the elicitation with ergosterol includes the mobilization of internal calcium stores in vacuoles mediated by IP3 and serine/threonine protein kinases.

Related projects

• Study of the effects of elicitins stimulating a defence reacions in plants.
• Genetic study of alcohol dependence : detection of the association between candidate gene polymorphisms and alcoholism.
• Modeling of the structure and interaction of elicitors of the fungus Phytophthora
• Biomolecular Structure-function Relationships and their role in the Metabolism