LIGNANS OF SCHISANDRA CHINENSIS: ANALYSIS IN PLANT CELL CULTURE AND ANTIPROLIFERATIVE ACTIVITY
| Authors | |
|---|---|
| Year of publication | 2003 |
| Type | Monograph |
| MU Faculty or unit | |
| Citation | |
| Description | Dibenzocyclo[a,c]diene lignans occur exclusively in genera Schisandra and Kadsura (Schisandraceae/Magnoliaceae). Schisnadra chinensis is well-known medicinal plants of traditional Chinese medicine in which fruits have been used as tonic and antitusic. These lignans have been shown to possess a broad range of biological effects, including antivirral, antioxidative and hepatoprotective activities. Content of lignans in fruits of the plants is relatively low (~1 %) and is composed of at least thirty lignans. Moreover, contents of individual lignans considerably vary from plant to plant. Plant cell culture may represent a solution of the supply of these lignans. We determined the production of lignans in cell culture of S. chinensis using HPLC. Our preliminary results showed, that amount of lignans in plant cell cultures was lower than that in fruits and leaves. The main lignan in samples of cell cultures was deoxyschizandrin, its maximum yield reached 0.13 % based on biomass dry weight. The concentration of dexyschizandrin in plant cell culture was 10 times lower than that in fruits and 3 times lower than that in leaves. The antiproliferative activity of lignans against HeLa cells was evaluated using the neutral red uptake assay. The nonpolar lignans with S-biaryl configuration, gomisin N and wuwezisu C, were found to be more cytotoxic than schizandrin and gomisin A, somewhat polar lignans with R-biaryl configuration. Gomisin N displayed the highest cytotoxicity of the tested lignans with IC50 37 mg/L. To learn the possible effects of gomisin N on the architecture of cytoskeleton, actin fibers and microtubules were visualized using fluorescence staining. Exposure of the HeLa cells to gomisin N induced a reorganization of the cytoskeleton with the reduction of microtubules and aggregation of actin at the cell periphery |
| Related projects: |