Improving measurement precision using automated micro-axial tomography
| Authors | |
|---|---|
| Year of publication | 2003 |
| Type | Article in Proceedings |
| Conference | Biophysics of the Genome |
| MU Faculty or unit | |
| Citation | |
| Field | Use of computers, robotics and its application |
| Keywords | human genome structure; image analysis |
| Description | Distance measurement between small targets plays an important role in human genome research. The precision of such measurements depends on the quality of images, the localisation precision of the targets and the orientation of the line connecting the targets. In micro-axial tomography, the observed objects (cells) are fixed to a glass fibre, which can be rotated. By rotating the fibre the orientation of the measured targets can be changed. The relationship between the localisation precision and the orientation of the targets has been studied in simple simulations. In micro-axial tomography, two main approaches to measurement exist. Either one high-resolution image is reconstructed from the acquired tilted images and the measurements are performed on the single image or the images in the series are measured separately and the results are combined. Both approaches are compared and discussed here. |
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