Analýza telomer nádorových buněk technikami in situ

Warning

This publication doesn't include Faculty of Arts. It includes Faculty of Science. Official publication website can be found on muni.cz.
Title in English Telomeres Analysis of Tumor Cells by in situ Techniques
Authors

SKLENIČKOVÁ Marie FAJKUS Jiří

Year of publication 2003
Type Article in Periodical
Magazine / Source Časopis Lékařů českých
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords telomeres; FISH; fiber-FISH; PRINS
Description Stable telomere maintenance is essential for the indefinite cellular proliferation of germline and tumour cells. In most cases, telomere synthesis is performed by nucleoprotein enzyme complex of telomerase, that results in stabilisation of telomeres shortened <= 7 kb. Rarely, telomeres may be maintained via alternative (recombination-based) mechanism, which produces telomeres of heterogenous lengths (3-50 kb). Analysis of telomeres by in situ techniques, such as fluorescent in situ hybridisation (FISH) on metaphase spread or on extended DNA fibres (fiber-FISH) and Primed in situ labelling (PRINS), enables distinguish between these two mechanisms and to analyse individual telomeres in the given type of cells.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.