Review: Advances in Capillary Electrophoretically Mediated Microanalysis
| Authors | |
|---|---|
| Year of publication | 2003 |
| Type | Article in Periodical |
| Magazine / Source | Electrophoresis |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | Capillary electrophoresis Electrophoretically mediated microanalysis Enzyme Derivatization Review |
| Description | This survey gives an overview of recent developments in the use of capillary electrophoretic techniques for the in-line study of enzyme reactions and derivatization protocols. The article is divided into two parts: one concerning in-line enzyme reactions and one concerning in-capillary derivatization. The first part introduces electrophoretically mediated microanalysis (EMMA) and discusses and illustrates the different modes of EMMA. Next, a literature overview is provided, starting from 1996, and the investigated enzymes are classified into two tables based on the mode of engagement (i.e. continuous or transient) of the developed EMMA based assay. The second part starts with an introduction of the procedures and the nomenclature used in the area of in-line derivatization protocols based on EMMA. Reported derivatization procedures are discussed and classified in tables, divided in sections following the functional group that is derivatized. Finally, conclusion. |
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