Electrophoretically mediated microanalysis (Review)

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Authors	VYTISKOVÁ Soňa VAN DYCK Sigrid VAN SCHEPDAEL Ann HOOGMARTENS Jos GLATZ Zdeněk
Year of publication	2004
Type	Article in Periodical
Magazine / Source	Journal of Chromatography A
MU Faculty or unit	Faculty of Science
Citation
Field	Biochemistry
Keywords	Capillary electrophoresis; enzymes; EMMA
Description	This review describes the existing developments in the use of the capillary electrophoretic microanalytical technique for the in-line study of enzyme reaction, electrophoretically mediated microanalysis (EMMA). The article is divided into a number of parts. After an introduction, the different modes, basic principle, procedure, and some mathematical treatments of EMMA methodology are discussed and illustrated. The applications of EMMA for enzyme assay and for non-enzymatic determination are summarized into two tables. In addition to classical capillary electrophoresis (CE) instrument EMMA, special emphasis is given to a relatively new technique: EMMA on CE microchip. Finally, conclusions are drawn.
Related projects:	Application of capillary zone electrophoresis for the study of enzymes Biomolecular Structure-function Relationships and their role in the Metabolism