ZVÝŠENÁ EXPRESE VIMENTINU JAKO MARKER DIFERENCIACE MONOBLASTŮ BM2

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Title in English INCREASE IN VIMENTIN EXPRESSION AS A MARKER OF BM2 MONOBLAST DIFFERENTIATION
Authors

BENEŠ Petr ZAHRADNÍČKOVÁ Eva KONEČNÁ Hana ZDRÁHAL Zbyněk ŠMARDA Jan

Year of publication 2004
Type Article in Proceedings
Conference VIII. Pracovní setkání biochemiků a molekulárních biologů. Sborník abstraktů.
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords vimentin; monocyte/macrophage differentiation; marker; proteomics
Description BM2 monoblasts can be induced to terminal differentiation using phorbol ester TPA. In this study we described changes of proteom of BM2 cells undergoing TPA-induced differentiation. Using protein gel electrophoresis we detected 55 kDa protein in maturing BM2 cells. This protein was identified as vimentin by mass spectrometry MALDI-TOF. This conclusion was confirmed by western blotting. The level of vimentin also increases in BM2 cells treated with trichostatin A and in RA-treated BM2 derivatives overexpressing retinoid acid receptors RAR or RXR. Increased level of vimentin can be thus used as differentiation marker in BM2 cells.
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