ZVÝŠENÁ EXPRESE VIMENTINU JAKO MARKER DIFERENCIACE MONOBLASTŮ BM2

Warning

This publication doesn't include Faculty of Arts. It includes Faculty of Science. Official publication website can be found on muni.cz.

Title in English	INCREASE IN VIMENTIN EXPRESSION AS A MARKER OF BM2 MONOBLAST DIFFERENTIATION
Authors	BENEŠ Petr ZAHRADNÍČKOVÁ Eva KONEČNÁ Hana ZDRÁHAL Zbyněk ŠMARDA Jan
Year of publication	2004
Type	Article in Proceedings
Conference	VIII. Pracovní setkání biochemiků a molekulárních biologů. Sborník abstraktů.
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	vimentin; monocyte/macrophage differentiation; marker; proteomics
Description	BM2 monoblasts can be induced to terminal differentiation using phorbol ester TPA. In this study we described changes of proteom of BM2 cells undergoing TPA-induced differentiation. Using protein gel electrophoresis we detected 55 kDa protein in maturing BM2 cells. This protein was identified as vimentin by mass spectrometry MALDI-TOF. This conclusion was confirmed by western blotting. The level of vimentin also increases in BM2 cells treated with trichostatin A and in RA-treated BM2 derivatives overexpressing retinoid acid receptors RAR or RXR. Increased level of vimentin can be thus used as differentiation marker in BM2 cells.
Related projects:	Study of molecular mechanisms causing v-Myb suppression using proteomics-based approach The role of vimentin inb the monocyte/macrophage differentiation pathway Genomes and their functions