Examination of membrane protein expression in Paracoccus denitrificans by two-dimensional gel electrophoresis

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Authors

BOUCHAL Pavel KUČERA Igor

Year of publication 2004
Type Article in Periodical
Magazine / Source Journal of Basic Microbiology
MU Faculty or unit

Faculty of Science

Citation
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Field Biochemistry
Keywords Paracoccus denitrificans; proteome; two-dimensional gel electrophoresis
Description The well-known metabolic versatility of the soil bacterium Paracoccus denitrificans poses a challenge for the modern proteomic approaches. We describe here the improved conditions that allow good separations and quantitative analyses of hundreds of membrane or periplasmic proteins. As an example, the results of a screening for membrane proteins associated predominantly with aerobic or anaerobic (denitrifying) modes of growth are presented.
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