Variations in ITS of ribosomal DNA of Czech Armillaria species determined by PCR and high performance liquid chromatography

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Authors	LOCHMAN Jan MIKEŠ Vladimír ŠERÝ Omar JANKOVSKÝ Libor
Year of publication	2004
Type	Article in Periodical
Magazine / Source	Mycological Research
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	Armillaria; PCR; fungi
Description	We analysed 40 isolates of species A. borealis, A. cepistipes, A. gallica, A. mellea, A. ostoyae and A. tabescens mostly collected in Czech Republic by PCR-RFLP.Restriction fragments were analysed by ion-exchange high performance liquid chromatography that proved to be more useful, more sensible and less time consuming than classical electrophoresis on agarose gel.The cluster analysis based on the restrictions patterns of restriction endonucleases AluI and HinfI divided the analysed species into three groups.
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