The rapid identification of European Armillaria species from soil samples by nested PCR

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Authors

LOCHMAN Jan ŠERÝ Omar MIKEŠ Vladimír

Year of publication 2004
Type Article in Periodical
Magazine / Source FEMS Microbiology Letters
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords Armillaria; PCR; soil
Description New specific primers AR1 and AR2 were successfully used for the amplification of a specific part of internal transcribed spacer (ITS) of rDNA of Armillaria isolated from soil samples.The individual species were distinguished by restriction fragment length polymorphisms (RFLP) analysis with restriction endonucleases HinfI. For the first time, a method is described enabling the identification of Armillaria species within one day directly from soil samples without the need for previous isolation and cultivation of mycelium
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