Determination of Midazolam from Perfuse Medium as a Probe Substrate of Cytochrome P450 3A1/2 Activity in Rats

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Title in English	DETERMINATION OF MIDAZOLAM FROM PERFUSE MEDIUM AS A PROBE SUBSTRATE OF CYTOCHROME P450 3A1/2 ACTIVITY IN RATS
Authors	JUŘICA Jan DOSTÁLEK Miroslav TOMANDL Josef HADAŠOVÁ Eva
Year of publication	2004
Type	Article in Proceedings
Conference	ACTA UNIVERISTATIS PALACKIANAE OLOMUCENSIS FACULTAS RERUM NATURALIUM
MU Faculty or unit	Faculty of Medicine
Citation
Field	Pharmacology and pharmaceutical chemistry
Keywords	Midazolam; CYP-450
Description	The aim of the project was to develop HPLC method for determination of midazolam and its three metabolites, 1- and 4-hydroxymidazolam and 1,4-dihydroxymidazolam from perfuse rat liver medium. Hydroxylation of midazolam is used as a valuable tool for measurement of metabolic activity of the 3A1/2 isoform of cytochrome P-450 in rats (CYP3A1/2)This method is simple, rapid, sensitive and applicable for modeling and description of possible pharmacological interactions on rat CYP3A1/2 or it`s human orthologue CYP3A4/5, respectively.
Related projects:	Plasticity of Regulatory Mechanisms of the Central Nervous Systems