MIDAZOLAM JAKO SELEKTIVNÍ  MARKER PODRODINY 3A CYTOCHROMU P450; FARMAKOKINETICKÁ CHARAKTERISTIKA MIDAZOLAMU

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Title in English	Midazolam as a probe substrate of 3A subfamily CYP-450, pharmacological parameters of midazolam
Authors	JUŘICA Jan DOSTÁLEK Miroslav PISTOVČÁKOVÁ Jana MINAŘÍKOVÁ Veronika HANESOVÁ Mariana HADAŠOVÁ Eva TOMANDL Josef
Year of publication	2004
Type	Article in Proceedings
Conference	Sborník abstrakt, Medifórum
MU Faculty or unit	Faculty of Medicine
Citation
Field	Pharmacology and pharmaceutical chemistry
Keywords	Midazolam; CYP-450
Description	The aim of the project was to develop HPLC method for determination of midazolam and its three metabolites, 1- and 4-hydroxymidazolam and 1,4-dihydroxymidazolam from perfuse rat liver medium. Hydroxylation of midazolam is used as a valuable tool for measurement of metabolic activity of the 3A1/2 isoform of cytochrome P-450 in rats (CYP3A1/2)This method is simple, rapid, sensitive and applicable for modeling and description of possible pharmacological interactions on rat CYP3A1/2 or it`s human orthologue CYP3A4/5, respectively. In the second part of the project, there was performed pharmacokinetic study after i.v. administration of midazolam.
Related projects:	Plasticity of Regulatory Mechanisms of the Central Nervous Systems