Clustering of genes with similar regulation patterns during monocyte and granulocyte differentiation of HL-60 cells

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Authors

KOUTNÁ Irena JANSOVÁ Eva KRONTORÁD Petr SVOBODA Zbyněk PAVLÍK Tomáš BÁRTOVÁ Eva KOZUBEK Michal KOZUBEK Stanislav

Year of publication 2004
Type Article in Proceedings
Conference Biophysics of the Genome
MU Faculty or unit

Faculty of Informatics

Citation
Field Genetics and molecular biology
Keywords microarrayc
Description In the present study, a high density-microarrays of human cDNA containing 19,000 genetic elements were used to search for differences in gene expression of human promyelic leukaemia cell line HL-60 during monocyte (MD) and granulocyte differentiation (GD). For differentiation to monocytes (granulocytes), the TPA, 12-O-tetradecanoylphorbol-13-acetate (DMSO) were used, respectively. A total number of 4625 (4760) genes were found to be regulated during MD (GD). The genes have been divided into groups according to their kinetics of regulation (R-groups). The genes of the same group are not distributed randomly throughout the genome but form clusters on chromosomes (R-clusters). Thus, functionally interconnected genes are frequently localized near to each other on the DNA molecule. In addition, genes of R-groups of MD (GD), if projected to GD (MD) happen to be mostly found in one or several R-groups of the other type, although the kinetics of regulation may be different (e.g. group 9 of GD and group 12 of MD). However, in most cases similar patterns of regulation correspond to each other (15, 16 of GD correspond to 3, 4 of MD).
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