The role of calmodulin in cell cycle.

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Authors

HAVELKOVÁ Marie MENŠÍK Pavel UNGER Eberhard

Year of publication 2005
Type Article in Periodical
Magazine / Source Journal of APPLIED BIOMEDICINE
MU Faculty or unit

Faculty of Education

Citation
Field Genetics and molecular biology
Keywords Calmodulin; cell cycle; Ca2+; indirect immunofluorescence; anti-calmodulin antibody; rhodamine-conjugated phalloidin; cytoskeletal actin; karyokinesis;
Description Calmodulin is a small, highly conserved, Ca2+-binding protein present in all eukaryotic cells. It is one of the mediators of Ca2+ signals induced by extracellular stimuli. Several lines of evidence have suggested that calmodulin is essential for eukaryotic cell proliferation. The intracellular localization of protein calmodulin and cytoskeletal actin in cells of yeast strain Yarrowia lipolytica has been studied by indirect immunofluorescence using anti-calmodulin antibody and rhodamine-conjugated phalloidin. We have found that there exist relationships between cytoskeletal components and protein calmodulin. Results: 1) the accumulation of calmodulin dots at the regions of cell growth suggests that this protein is important for cell polarity development in yeast; 2) calmodulin emerges in mentioned parts of the cell always sooner than actin. It proves that calmodulin is required for distribution of actin structures; 3) calmodulin is unconditionally needed for nuclear division.
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