PROTEOMIC STRATEGY FOR INVESTIGATIGATION OF CYTOKININ ACTION IN ARABIDOPSIS THALIANA

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Authors

BÖHMOVÁ Gabriela ZDRÁHAL Zbyněk KONEČNÁ Hana KOUKALOVÁ Šárka BRZOBOHATÝ Břetislav

Year of publication 2005
Type Article in Proceedings
Conference Molecular and cellular proteomics
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords proteomics; mass spectrometry; plant hormone
Description Proteomics-based approach for the investigation of Arabidopsis thaliana seedlings with cytokinin overproduction was applied. We used both MALDI-MS and LC-MS/MS techniques for peptide analysis. Our results showed the effective application of different methods for protein identification. In our case the combination of MALDI-MS and LC-MS/MS increased the number of identified proteins to over 90 % of all analysed samples.
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