Size and shape of mycoplasma virus MV-Lg-L 172 DNA

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Authors	DRÁŠIL V. DOŠKAŘ Jiří VIKLICKÁ S. TKADLEČEK L.
Year of publication	1978
Type	Article in Periodical
Magazine / Source	Acta virologica
MU Faculty or unit	Faculty of Science
Citation
Web	http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=35941
Field	Microbiology, virology
Keywords	mycoplasma virus
Description	Sedimentation of DNA of mycoplasma virus MV-Lg-L 172 in neutral and alkaline density gradients showed that the chromosome of this virus is most probably formed by a double-stranded circular DNA molecule with a molecular weight of approx. 10(7) daltons. These results were confirmed by direct electron microscopy of the viral DNA. Gamma-irradiation of the virus caused in its DNA single breaks on the average per each 66 eV of absorbed energy. Virus survival depended exponentially on the irradiation dose, D0 being between 60,000 and 70,000 rads. This dose induced on the average one single break in the DNA
Related projects:	Genomes and their functions