Intra- and extraneuronal changes of immunofluorescence staining for inflammation-related molecules in the experimental models of peripheral neuropathic pain
| Title in English | Intra- and extraneuronal changes of immunofluorescence staining for inflammation-related molecules in the experimental models of peripheral neuropathic pain. |
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| Authors | |
| Year of publication | 2005 |
| Type | Article in Proceedings |
| Conference | Folia Medica Cassoviensia |
| MU Faculty or unit | |
| Citation | |
| Field | Neurology, neurosurgery, neurosciences |
| Keywords | dorsal root ganglia; TNF-alpha; TNFR1 |
| Description | Peripheral neuropathic pain has been investigated using rat peripheral nerve injury models including unilateral sciatic nerve transection or ligation, spinal nerve ligation or ventral root transection. The experimental models were utilized for the study of immunofluorescence (IF) staining for IL-1b, IL-6, TNFa and TNFaR1 in the rat DRG. Quantitative and qualitative IF changes in the DRG affected by unilateral nerve injury were compared with the contralateral ones removed from operated animals as well as naive rats. Ipsi- and contralateral DRG displayed increased IF for TNFa and TNFaR1 when compared with DRG of naive rats. The TNFa IF was increased in both DRG neurons and their satellite glial cells, while enhanced IF for TNFaR1 was observed only at the surface of satellite glial cells after various types of nerve injury. In addition, ipsilateral DRG exhibited an elevation of TNFa immunoreactivity by invaded ED-1+ macrophages. The IL-1b IF was increased in the DRG neurons following sciatic nerve constriction, but not after the nerve trasection. A higher level of IF for IL-6 was observed in the DRG neurons and their satellite glial cells following sciatic nerve ligature as well as ventral root transection. |
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