Cell Line RAW264.7 as a model for osteoclastogenesis and methods of its genetic manipulation

• Authors: VAŇHARA Petr; KAUCKÁ Markéta; MACEČKOVÁ Vendula; ŠMARDA Jan
• Year of publication: 2006
• Type: Article in Proceedings
• Conference: X. pracovní setkání biochemiků a molekulárních biologů
• MU Faculty or unit: Faculty of Science
• Field: Oncology and hematology
• Keywords: differentiation; osteoclast; transfection
• Description: We performed a comparative study of several conceivable transfection methods to prepare stable transfectants of RAW264.7 cells. These cells are intended to be used to address the function of certain genes coding for transcription foctors in differentiation and activation of osteoclasts.

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