Electrochemical immunosensor for detection of Francisella tularensis

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Authors

SKLÁDAL Petr SYMERSKÁ Yveta POHANKA Miroslav ŠAFÁŘ Bohuslav MACELA Aleš

Year of publication 2005
Type Chapter of a book
MU Faculty or unit

Faculty of Science

Citation
Description The immunosensor for rapid and sensitive detection of Francisella tularensis (Ft) was developed using screen-printed sensors with multiple (4) Au working electrodes. The sensing zones were modified with a biolayer of anti-Ft polyclonal antibody attached through the covalently immobilized Protein A. The sandwich assay format was employed with anti-Ft IgG-peroxidase conjugate as tracer. For electrochemical measurements, an automated portable detector was developed; it consisted of a digitally controlled potentiostat and four embedded miniperistaltic pumps. The flow-through measurement involved sequential incubations with sample (10 min), tracer (10 min) and substrate mixture (iodide and hydrogen peroxide). The enzymatically-produced iodine was detected electrochemically at - 50 mV. The limit of detection was 100 Ft cells/ml; the response was significantly higher (3x) when using the living cells compared to the heat inactivated ones.
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