Determination of midazolam and its metabolites in rat plasma and perfuse medium by HPLC

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Authors

JUŘICA Jan TOMANDL Josef DOSTÁLEK Miroslav HADAŠOVÁ Eva

Year of publication 2006
Type Article in Proceedings
Conference CD of the abstracts of the 29th international symposium on capillary chromatography
MU Faculty or unit

Faculty of Medicine

Citation
Field Biochemistry
Keywords Cytochrome P450; Midazolam; 1 Hydroxymidazolam; 4 Hydroxymidazolam; 1 4 Dihydroxymidazolam; Rat; Perfuzate; Plasma
Description A new isocratic reverse-phase high-performance liquid chromatography utilising ultraviolet detection for the simultaneous determination of midazolam and its three main hydroxymetabolites in rat plasma and rat liver perfuse medium is described. The present method offers various advantages over the currently used HPLC methods, including speed, sensitivity and reproducibility. Moreover, it may be applicable for modelling and description of possible pharmacological interactions on rat or human isoforms of cytochrome P450.
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