Determination of midazolam and its metabolites in rat plasma and perfuse medium by HPLC

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Authors	JUŘICA Jan TOMANDL Josef DOSTÁLEK Miroslav HADAŠOVÁ Eva
Year of publication	2006
Type	Article in Proceedings
Conference	CD of the abstracts of the 29th international symposium on capillary chromatography
MU Faculty or unit	Faculty of Medicine
Citation
Field	Biochemistry
Keywords	Cytochrome P450; Midazolam; 1 Hydroxymidazolam; 4 Hydroxymidazolam; 1 4 Dihydroxymidazolam; Rat; Perfuzate; Plasma
Description	A new isocratic reverse-phase high-performance liquid chromatography utilising ultraviolet detection for the simultaneous determination of midazolam and its three main hydroxymetabolites in rat plasma and rat liver perfuse medium is described. The present method offers various advantages over the currently used HPLC methods, including speed, sensitivity and reproducibility. Moreover, it may be applicable for modelling and description of possible pharmacological interactions on rat or human isoforms of cytochrome P450.
Related projects:	The internal organisation and neurobiological mechanisms of functional CNS systems under normal and pathological conditions. Capillary electrophoresis as a tool for the drug-metabolism studies Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie