Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein

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Authors	KOCÁBEK Tomáš ŘEPKOVÁ Jana DUDOVÁ Markéta HOYEROVÁ Klára VRBA Lukáš
Year of publication	2006
Type	Article in Periodical
Magazine / Source	Genetica
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	Arabidopsis thaliana - embryonic defect - mitochondria - pentatricopeptide repeat - semi-lethality - T-DNA mutagenesis
Description	A novel Arabidopsis thaliana mutant of one member of the PPR (pentatricopeptide repeat) gene family has been identified among T-DNA insertion lines. Tagging of the At1g53330 gene caused the appearance of a semi-lethal mutation with a complex phenotypic expression from embryo lethality associated with the abnormal pattern of cell division during globular to heart transition to fertile plants with just subtle phenotypic changes. The PPR protein At1g53330.1 was predicted to be targeted to mitochondria by TargetP and MitoProt programs. Complementation analysis comfirmed that phenotype is a result of a single T-DNA integration. A thorough functional analysis of this mutant aimed at finding a particular organelle target of At1g53330.1 protein will follow.
Related projects:	Molecular basis of cell and tissue regulations Developmental and Evolutionary Genetics of Plants Genomes and their functions