Species identification of Bifidobacterium strains isolated from the stool samples os sucking babies

Warning

This publication doesn't include Faculty of Arts. It includes Faculty of Science. Official publication website can be found on muni.cz.

Authors	ŠPANOVÁ Alena RITTICH Bohuslav KŘÍŽOVÁ Jana KŠICOVÁ Kateřina
Year of publication	2006
Type	Article in Proceedings
Conference	5th Meeting of the European Mucosal Immunology Group
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	Bifidobacterium; sucling babies; ARDRA; restriction endonucleases;
Description	Molecular biological methods based on species-specific PCR, and amplified ribosomal DNA restriction analysis (ARDRA) of PCR amplicons using 6 restriction enzymes were used to the identification and characterization of new Bifidobacterium strains isolated from the stool samples of suckling babies. Identification of strains of human origin was succesfull in 80% of strains. Bifidobacteria were identified into 6 species with prevalence of B. longum, B. catenulatum/B. pseudocatenulatum, B. bifidum, B.animalis/B.lactis, B.breve and B. adolescentis. Altogether 20% of new isolated strains could not be identified sofar due to the cross reactions or no amplicons detetection. Occurence of unknown species of Bifidobacteria can be considered.
Related projects:	Molecular basis of cell and tissue regulations Introduction of new traits for quality and stability improving in variety production of selcted leguminous species by hybridization methods The use of procedures on basis of molecular genetics in acquiring of new bacterial strains with probiotic properties with the aim to increase specific genetic sources for production of functional foods