Automatická mikroskopie a poziční klonování v přístupech přímé genetiky.
| Title in English | Automated microscopy and map-based cloning in the forvard genetics aproaches |
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| Authors | |
| Year of publication | 2006 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | The forward genetics approaches were in the past applied as a basic tool in the identification of genetically determined traits in all organisms. Knowledge of all genomes of model organisms allowed the use of new approaches, mainly the reverse genetics as a very effective tool in the identification of function of many genes. However, the introduction of new methods into the approaches of forward (classical) genetics allowed to significantly increase a rate and to simplify the process of the gene identification. This might allow to combine the advantages of the forward genetics, i.e. the phenotype-based selection with molecular methods, employing molecular markers, allowing thus to monitor broad spectrum of different biological processes on molecular level. To eliminate the constraints of the rate-limiting factor that is a microscopy analysis of mutant lines, we have adopted automated microscopy. The .slide system allows us to screen up to 50-100 lines a day. The lines with identified changes in the expression pattern of used marker genes (e.g. uidA and/or GFP) will then be analyzed and affected genes identified using map based cloning approaches with the use of molecular markers accessible in the public available databases (TAIR and Monsanto (Cereon Genomics)). Supported by MSM0021622415 a LC06034. |
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