pOp6/LhGR: a stringently regulated and highly responsive dexamethasone-inducible gene expression system for tobacco.
| Authors | |
|---|---|
| Year of publication | 2005 |
| Type | Article in Periodical |
| Magazine / Source | The Plant Journal |
| MU Faculty or unit | |
| Citation | |
| Field | Botany |
| Keywords | chemically inducible gene expression; cytokinin-biosynthetic gene ipt; tobacco |
| Description | pOp/LhGR, a dexamethasone-inducible derivative of the pOp/LhG4 transcription activation system, and its use in tobacco to regulate expression of uidA (encoding beta-glucuronidase; GUS) and the cytokinin-biosynthetic gene ipt is described. The pOp/LhGR system exhibited stringent regulation and strong induced phenotypes in soil and tissue culture. A single dose of dexamethasone was sufficient to direct cytotoxic levels of ipt expression in soil grown plants although uninduced plants grew normally throughout a complete life cycle. In vitro, induced transcripts were detectable within an hour of dexamethasone application and 1nM dexamethasone was sufficient for half maximal induction of GUS activity. Various methods of dexamethasone application were successfully applied under tissue culture and greenhouse conditions. No inhibitory effects of dexamethasone or LhGR on plant development even with the highest concentrations of inducer were observed. |
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