Double elimination of oligonucleotides

• Authors: MIKELOVÁ Radka; TRNKOVÁ Libuše; JELEN František
• Year of publication: 2007
• Type: Article in Proceedings
• Conference: Sborník příspěvků VII. Pracovního setkání fyzikálních chemiků a elektrochemiků
• MU Faculty or unit: Faculty of Science
• Field: Electrochemistry
• Keywords: Elimination voltammetry; Double elimination voltammetry;electrochemistry; Oligonucleotides
• Description: Ten years ago the theory of elimination voltammetry with linear scan (EVLS) was published and experimentally verified for reversible, quasi-reversible and irreversible electrode systems1-3. The EVLS can be considered a mathematical model of the transformation of current-potential curves capable of eliminating some selected current components, and conserving the other ones. This contribution is devoted to the EVLS transformation of irreversible current-potential curves of an adsorbed electroactive substance. The function eliminating charging and kinetic current components, and conserving the diffusion current component, yields specific, sensitive and well developed peak-couterpeak (p-cp) signal3-5. Theoretical value of ratio Ip/(Ip+Icp) is 0,40973,5,8. This signal was successfully employed in the analysis of nucleic acids and short homo- or hetero-deoxyoligonucleotides (ODNs) containing adenine (A) and cytosine (C)4-12.

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