Determination of asymmetric dimethylarginine in human plasma by HPLC with coulometric detection

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Authors

KLEPÁRNÍK Martin TOMANDL Josef

Year of publication 2007
Type Article in Proceedings
Conference HPLC 2007. Abstract Book.
MU Faculty or unit

Faculty of Medicine

Citation
Web 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques - HPLC 2007
Field Analytic chemistry
Keywords asymmetric dimethylarginine; HPLC; coularray detection
Description Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. ADMA is a competitive inhibitor of nitric oxide synthase. We report here development of a new HPLC method for the determination of ADMA using coulometric detection. After solid-phase extraction procedure on a strong cation exchange column and evaporation of the eluate under gentle stream of nitrogen at 70 to 75 C the reconstituted samples were derivatised with ortho-phthaldialdehyde reagent containing 3-mercaptopropionic acid. Formed derivatives were analysed on a reversed phase column Hydro-RP using isocratic elution and coulometric detection.
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