Multidetection Platform for Microcolumn Separation of Proteins and Peptides

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Authors	PREISLER Jan KRÁSENSKÝ Pavel VRÁBEL Patrik PEŠ Ondřej VACULOVIČ Tomáš FOLTYNOVÁ Pavla VYHNÁNEK Radek
Year of publication	2007
Type	Article in Proceedings
Conference	Book of Abstracts and Abbreviated Papers 13th ISSS & 13th AACI
MU Faculty or unit	Faculty of Science
Citation
Field	Analytic chemistry
Keywords	microcolumn; separation; mass spectrometry; MALDI; CE; HPLC; fluorescence; LIF; inductively-coupled plasma
Description	The lecture describes an instrumentation platform based on a universal interface for deposition of CE or microLC eluent on a target of a MALDI mass spectrometer. The deposited fractions of proteins or peptides can be analyzed in off-line mode using MALDI mass or tandem mass spectrometry (MS or MS/MS) and native laser-induced fluorescence (LIF) detection or subjected to on-target reactions, such as digestion for protein identification.
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