Identifikace a stanovení příbuznosti probiotických druhů bakterií rodu Bifidobacterium pomocí molekulárně diagnostických metod

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Title in English	Identification and determination of relatioships of Bifidobacterium probiotic strains using molecular diagnostic methods
Authors	KRULOVÁ Blanka ŠPANOVÁ Alena RITTICH Bohuslav
Year of publication	2007
Type	Article in Proceedings
Conference	XI. Setkání biochemiků a molekulárních biologů
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	Bifidobacterium; PCR; rep-PCR
Description	The aim of the work was the use of species specific PCRs for the identification of bacterial species of genus Bifidobacterium isolated from stool of suckling babies. The other aim was the use of rep-PCR for PCR/fingerprinting of studied strains. Species specific PCRs were carried out with 10 different primer pairs. All isolates were indentified as of genus Bifidobacterium, 3 isolates were identified as species B. bifidum, 3 isolates were identified as species B. animalis, 6 isolates were identified as species B. breve, 2 isolates were identified as species B. adolescentis. Other molecular biology methods will be used for confirmation of the above mentioned results
Related projects:	Molecular basis of cell and tissue regulations The use of procedures on basis of molecular genetics in acquiring of new bacterial strains with probiotic properties with the aim to increase specific genetic sources for production of functional foods