Využití metody ARDRA pro druhovou identifikaci nových kmenů bakterií rodu Bifidobacterium

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Title in English	The use of ARDRA method for specific identification of new Bifidobacterium strains
Authors	KŠICOVÁ Kateřina ŠPANOVÁ Alena RITTICH Bohuslav
Year of publication	2007
Type	Article in Proceedings
Conference	XI. Setkání biochemiků a molekulárních biologů
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	Bifidobacterium; ARDRA; identification
Description	ARDRA analysis was used for the identification of 11 selected bacterial strains isolated from human sources. These strains were identified into the genus Bifidobacterium using genus-specific PCR. However they could not be identified into species using species-specific PCR due to the cross reactions. Analysis of genus specific amplicon of 914 bp using restrictases AluI and Sau3AI enabled the differentiation of species B. adolescentis, B. bifidum, B. breve, B. catenulatum, B. infantis and B. longum. Alltogether 10 strains could be identified into species. In conclusion, ARDRA analysis is a quick and reproducible identification procedure with high discrimination potential for individual species.
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