Electrochemical Sensing of Chromium-Induced DNA Damage: DNA Strand Breakage by Intermediates of Chromium(VI) Electrochemical Reduction.
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Periodical |
| Magazine / Source | ELECTROANALYSIS |
| MU Faculty or unit | |
| Citation | |
| Web | http://loschmidt.chemi.muni.cz/peg/pdf/electroanalysis07.pdf |
| Field | Biophysics |
| Keywords | Electrochemical Sensing of Chromium-Induced DNA Damage: DNA Strand Breakage; Intermediates of Chromium(VI) ; Electrochemical Reduction. |
| Description | DNA damage by CrV and/or CrIV intermediates of CrVI electrochemical reduction was detected using a supercoiled DNA-modified mercury electrode. A signal sensitive to formation of DNA strand breaks, AC voltammetric DNA peak 3, increased due to incubation of the DNA-modified electrode in micromolar solutions of CrVI at potentials sufficiently negative for CrVI reduction. Damage to DNA in solutions containing CrVI and a chemical reductant (ascorbic acid, AA) was observed only at relatively high chromium concentrations (hundreds of M). To eliminate interferences of excess CrVI in measurements of guanine electrochemical signals, a magnetoseparation double surface electrochemical technique was introduced. Using this approach, DNA damage in solution was detected for 50-250 M CrVI upon addition of 1 mM AA. Our results suggest a more efficient DNA damage at the electrode surface due to continuous production of the reactive chromium species, compared to DNA exposure to chromium being reduced chemically in solution. |
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