Methods and results of pharmacokinetic research at the departmentof pharmacology, faculty of medicine, Masaryk University, Brno, Czech republic
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| Year of publication | 2007 |
| Type | Article in Proceedings |
| Conference | Biomedical Papers 151 |
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| Keywords | research; results; pharmacokinetics; |
| Description | Research in the field of pharmacokinetic at the Department of Pharmacology, Faculty of Medicine, Masaryk University in Brno is a part of all granted research projects focused a long time on drugs influencing the CNS functions especially those biodegraded by the isoenzymes of the cytochrome P 450 (CYP). Both, pharmacokinetic studies in vivo as well as in vitro model of isolated and perfused rat liver is used. New HPLC and electrophoretic methods for determination of specific markers of metabolic activity for selected CYP isoenzymes are developed. Recent results received during last 3 years of rat studies both in vitro and in vivo showed stimulant interaction potential of methamphetamine with isoenzymes 1A1/2, CYP2C6/11, CYP2D2 a CYP3A4/5 which are involved in metabolism of clinically used drugs of many classes. Gender studies confirmed differential bioactivity and sensitivity to changes of isoenzymes analysed in male and female rats. There was also found significantly important effect of St John's wort (Hypericum perforatum) extract LI 160 used in the indication of depression on CYP2C6 cytochrome P-450 activity in perfused rat liver. In collaboration with psychiatrists are performed clinical studies evaluating patient's phenotype of metabolic enzymes for treatment with psychotropic drugs. Results refer for instance to great importance of CYP2D6 isoenzyme relation to efficacy of antidepressants prescribed in patients suffering from mental anorexia and sexual dysfunctions, and to some extent with gender differences. The team devoted to pharmacokinetic research published Czech textbook on pharmacokinetic principles. |