Nový protein rhodanasového typu u Acidithiobacillus ferrooxidans - funkční studie

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Title in English New rhodanese-like protein from Acidithiobacillus ferrrooxidans - functional study
Authors

HOUSER Josef JANICZEK Oldřich WIMMEROVÁ Michaela

Year of publication 2008
Type Article in Proceedings
Conference Chem. Listy 102, 365–404 (2008) - VIII. Mezioborové setkání mladých biologů, biochemiků a chemiků
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords Adidithiobacillus ferrooxidans; rhodanese; activity
Description The aim of this study is rhodanese group member - protein RHC. It is the only enzyme in Acidithiobacillus ferrooxidans genome that contains both rhodanese and ankyrin domain. There is no such an enzyme characterized so far. Our work can thus bring new informations useful not only for A. ferrooxidans study. After the optimization of expression and purification, there were performed the basic functional analysis, including pH and temperature optimum, catalytical konstants (Km) determination and other properties.
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