Comparison of PCR-ready DNA Isolation Methods from Lactic Acid Bacteria used in Dairy Industry

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Authors

ŠPANOVÁ Alena RITTICH Bohuslav NĚMCOVÁ Eva KŘÍŽOVÁ Jana HORÁK Daniel

Year of publication 2008
Type Article in Proceedings
Conference 7th International Conference on the Scientific and Clinical Applications of Magnetic Carriers
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords DNA; isolation; magnetic microspheres; lactic acid bacteria; PCR
Description Different methods for isolation of PCR-ready DNA were tested. The influence of the DNA isolation procedures tested on the reproducibility of RAPD and rep-PCR was studied. The reproducibility of amplification of DNA isolated by the salting-out method and adsorption on magnetic nonporous microspheres was comparable with the phenol extraction method.
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