Přínos fenotypizace plazmocytů v diagnostice mnohočetného myelomu a monoklonální gamapatie nejasného významu
| Title in English | Plasma cells phenotypisation in diagnostics of multiple myeloma and monoclonal gammopathy of undetermined significance |
|---|---|
| Authors | |
| Year of publication | 2008 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Monoclonal gammopathy of undetermined significance (MGUS) is an asymptomatic plasma cells (PCs) disorder, which can eventually progress into malignant multiple myeloma (MM). Multicolour flow cytometry is a sensitive tool for the analysis of number and clonality of PCs in these monoclonal gammopathies. The aim of this study was to analyse plasma cells (PCs) in MGUS and MM patients and to find parameter which can distinguish between them. PCs were identified as CD38+CD138+ cells and expression of CD19 and CD56 was used for discrimination of normal (N-PCs) and abnormal (A-PCs) PCs, respectively. Analysis of light chain restiction was done in selected cases. Flow cytometry is a sufficiently sensitive method for analysis of N-PCs (CD19+CD56- PCs) and abnormal A-PCs (CD56+CD19-, CD56+CD19+ or CD56-CD19-), even in low infiltration of bone marrow. Relative number of CD19+ PCs is an useful parameter for discrimination of MGUS from all clinical stages of MM. |
| Related projects: |