Metody klasické a molekulární cytogenetiky v diagnostice mnohočetného myelomu
| Title in English | Rele of classical and molecular cytogenetics for diagnosis of multiple myeloma |
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| Authors | |
| Year of publication | 2008 |
| Type | Article in Periodical |
| Magazine / Source | Klinická onkologie |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | Chromosomal aberrations; cytogenetics; FISH; multiple myeloma |
| Description | Chromosomal abnormalities are one of the most important and independent prognostic factor in patients with multiple myeloma. Classical cytogenetics based on analysis of mitotic figures is difficult due to the low proliferative index of malignant plasma cells. In order to circumvent this pitfall, molecular cytogenetics methods especially interphase fluorescence in situ hybridization (FISH) with centromeric or locus specific DNA probes is recently used to analyze the most frequent numerical and structural chromosomal abnormalities observed in myeloma. The main difference with leukemias is that interphase FISH in myeloma should be associated with plasma cell recognition in the bone marrow specimens. The aim of this article is to summarize the principles, values and limits of classical and molecular cytogenetic methods and their modifications used for the routine identification of cytogenetic abnormalities in multiple myeloma patients. The most common types of chromosomal aberrations found in karyotype of multiple myeloma patients and their prognostic importance are presented. |
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