Gene Expression differences in bone marrow hematoietic stem cell subpopulation

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Authors	KOUTNÁ Irena KOUTNÁ Irena KLABUSAY Martin PETERKOVÁ Martina HRABČÁKOVÁ Viera POTĚŠILOVÁ Michaela KRONTORÁD Petr KOZUBEK Michal
Year of publication	2008
Type	Conference abstract
MU Faculty or unit	Faculty of Informatics
Citation
Description	The gene expression profile of different hematopoietic stem cell (HSCs) subpopulations and the evaluation of correlation with their biological function is still poorly described problem. In order to bring a new information to this very important research area we used cDNA large-scale (19200 ESTs) microarrays technology for expression profiling of three hematopoietic stem cells enriched populations of human bone marrow. In our experiments we evaluated bone marrow HSCs obtained using the following three methods. (i) FACS CD34+ separation, (ii) immunomagnetic CD34+ separation and (iii) Lin- immunomagnetic separation.
Related projects:	Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies