The effect of bortezomib on myeloma cell line
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| Year of publication | 2009 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Introduction: Multiple myeloma (MM) is still an incurable disease characterized by the clonal expansion of malignant plasma cells. New anticancer drugs further improve prognosis of myeloma patients. The aim of this study was to evaluate changes in protein expression of myeloma cell line ARH 77 after bortezomib treatment. Materials and methods: Myeloma cell line ARH 77 was treated with bortezomib (10 – 40nM) for various periods of time (24 and 48 hours). The proteins contained in total myeloma cell lysate were separated by 2-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and the differentially expressed proteins between the untreated and treated cell lines were excised and identified by mass spectrometry. Results: There were analyzed 94 proteins differentially expressed between treated and control cells; total of 34 protein spots were upregulated: proteins involved in regulation of apoptosis, chaperons/stress related proteins, proteolysis of ubiquitin/protein degradation and cytoskeleton proteins. Sixty protein spots were downregulated: proteins involved in synthesis, regulation of apoptosis, chaperons/stress related proteins, regulation of cell cycle proteins, proteins connected to glycolysis and proteolysis of ubiquitin/protein degradation and antioxidant/redox proteins. Conclusion: We identified 94 proteins altered in myeloma cells after various exposure of to bortezomib. This proteomic approach can contribute to elucidation of mechanisms of new anticancer drugs action. |
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