VYUŽITÍ DETEKCE CYTOKINŮ PŘI PŘÍPRAVĚ VAKCÍN PROTI

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Title in English ROLE OF CYTOKINE QUANTIFICATION IN PREPARATION OF CELLULAR
Authors

JELÍNEK Jiří OČADLÍKOVÁ Darina MIKULKOVÁ Zuzana HÁJEK Roman MICHÁLEK Jaroslav

Year of publication 2008
Type Article in Periodical
Magazine / Source Klinická onkologie
MU Faculty or unit

Faculty of Medicine

Citation
Field Oncology and hematology
Keywords dendritic cells; multiple myeloma; interleukine 12; interleukine 10; ELISA; Cytometric Bead Array
Description Cytokines mediate intercellular interactions and their quantifi cation represents an important instrument of modern immunology and cell biology used for studies of cell-to-cell communication, including an immune response. This study demonstrates two ways of heterodimeric p70 interleukin 12 (IL-12p70) and IL-10 measurement using conventional ELISA and the novel and highly sensitive Cytometric Bead Array method during the preparation of clinical grade dendritic cell-based vaccine for patients with multiple myeloma.
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