Biomarker discovery in low-grade breast cancer using isobaric stable isotope tags and two-dimensional liquid chromatography-tandem mass spectrometry (iTRAQ-2DLC-MS/MS) based quantitative proteomic analysis

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Authors	BOUCHAL Pavel ROUMELIOTIS Theodoros HRSTKA Roman NENUTIL Rudolf VOJTĚŠEK Bořivoj GARBIS Spiros D.
Year of publication	2009
Type	Article in Periodical
Magazine / Source	Journal of Proteome Research
MU Faculty or unit	Faculty of Science
Citation
Doi	http://dx.doi.org/10.1021/pr800622b
Field	Biochemistry
Keywords	Breast cancer; biomarkers; proteomics; mass spectrometry; liquid chromatography; iTRAQ
Description	The present pilot study constitutes a proof-of-principle in the use of a quantitative LC-MS/MS based proteomic method for the comparative analysis of representative low-grade breast primary tumor tissues with and without metastases and metastasis in lymph node relative to the nonmetastatic tumor type. The study method incorporated iTRAQ stable isotope labeling, two-dimensional liquid chromatography, nanoelectrospray ionization and high resolution tandem mass spectrometry using the hybrid QqTOF platform (iTRAQ-2DLC-MS/MS). The principal aims of this study were (1) to define the protein spectrum obtainable using this approach, and (2) to highlight potential candidates for verification and validation studies focused on biomarkers involved in metastatic processes in breast cancer. The study resulted in the reproducible identification of 605 nonredundant proteins.
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