The first occurrence of the cyanobacterial alkaloid toxin cylindrospermopsin in the Czech Republic as determined by immunochemical and LC/MS methods

Warning

This publication doesn't include Faculty of Arts. It includes Faculty of Science. Official publication website can be found on muni.cz.

Authors	BLÁHOVÁ Lucie ORAVEC Michal MARŠÁLEK Blahoslav ŠEJNOHOVÁ Lenka ŠIMEK Zdeněk BLÁHA Luděk
Year of publication	2009
Type	Article in Periodical
Magazine / Source	Toxicon
MU Faculty or unit	Faculty of Science
Citation
Field	Environment influence on health
Keywords	cyanotoxin;cylindrospermopsin;methods of detection
Description	We report comparison of the immunochemical ELISA determination with the liquid chromatography/ mass spectrometry (LC/MS) method for CYN analyses in the samples from the Czech Republic, Central Europe. Results of the ELISA and LC/MS showed good qualitative agreement. However, concentrations determined by ELISA were systematically higher than concentrations determined by LC/MS. Differences between methods could be attributed to matrix effects (both in ELISA and LC/MS) or ELISA cross-reactivity with other unidentified derivatives of CYN. Our study demonstrates for the first time occurrence of CYN in the Central Europe.
Related projects:	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL) Tumor promotional mechanisms of cyanobacterial metabolites