Dependence of adenine isolation efficiency on the chain length evidenced using paramagnetic particles and voltammetry measurements
| Authors | |
|---|---|
| Year of publication | 2009 |
| Type | Article in Periodical |
| Magazine / Source | JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS |
| MU Faculty or unit | |
| Citation | |
| Field | Electrochemistry |
| Keywords | Paramagnetic particles; Adenine; Adsorptive transfer stripping technique; Square wave voltammetry |
| Description | The main aim of this work was to study the dependence of oligo adenine isolation efficiency on the chain length by using paramagnetic particles covered by homo eoxythymidines ((dT)25) with subsequent detection by adsorptive transfer technique coupled with square wave voltammetry. For this purpose, the oligonucleotides of the length A5, A10, A15, A20, A25, A30, A35, A40 and poly(A) in various concentrations were chosen. We determined that the isolation efficiency defined as "isolated oligonucleotide concentration''/"given oligonucleotide concentration" was about 55% on average. Sequence A25 demonstrated the best binding onto microparticles surface. (c) 2009 Elsevier B.V. All rights reserved. |
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