Chromatin dynamics during DSB repair
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Periodical |
| Magazine / Source | Biochimica et Biophysica Acta, Molecular Cell Research |
| MU Faculty or unit | |
| Citation | |
| Field | Biophysics |
| Keywords | chromatin structure; DNA damage; double-strand breaks (DSB); DNA repair; exchange aberration; genomic instability |
| Description | We show that double strand breaks (DSBs) induced in chromatin of low as well as high density by exposure of human cells to gama-rays are repaired in low-density chromatin. Only slight movement of sporadic DSB loci for short distances was noticed in living cells associated with chromatin decondensation around DSBs. This frequently resulted in their protrusion into the low-density chromatin domains. In these regions, the clustering (contact or fusion) of DSB foci was seen in vivo, and in situ after cell fixation. We propose that the repair of DSB in clustered foci might lead to misjoining of ends and, consequently, to exchange aberrations. |
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