Phosphopeptides analysis using enrichment techniques and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
| Authors | |
|---|---|
| Year of publication | 2009 |
| Type | Article in Proceedings |
| Conference | 3rd Central and Eastern European Proteomics Conference. Book of Abstracts |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | proteomic analysis; phosphoproteome |
| Description | In the present study, two methods for enrichment of tryptic phosphopeptides were described: IMAC and titanium dioxide chromatography. After enrichment procedure the phosphopeptides were analyzed by MALDI-TOF MS. Alpha-casein was used as model phosphoprotein. The methods were applied to protein mixture of Arabidopsis thaliana. |
| Related projects: |