Detection of metallothionein and alpha-methylacyl CoA racemase as potential new markers for prostate carcinoma
| Authors | |
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| Year of publication | 2009 |
| Type | Article in Periodical |
| Magazine / Source | International Journal of Molecular Medicine |
| MU Faculty or unit | |
| Citation | |
| Field | Oncology and hematology |
| Keywords | prostate carcinoma; tumor markers; metallothionein; AMACR |
| Description | In many developed countries, prostate cancer is the second leading cause of cancer-related deaths among men. Therefore, having reliable and easy-to-detect marker is needed to treat this disease successfully. Metallothioneins (MT) are a group of proteins rich in cysteine with molecular weights ranging from 6 to 10 kDa. Their main physiological functions are homeostatic control and detoxification of the metals. The role of MT in tumour tissue remains still unclear, but MT can be considered as new promising tumour marker. Besides MT, alpha-methylacyl CoA racemase AMACR has been proven to be one of the few biomarkers that can help distinguish cancer from benign cells, with high sensitivity and specificity for prostate carcinoma. In this study we proposed new methodical approaches in MT and AMACR detection in cell line model and then reciprocal correlation of obtained data. The LNCaP cell line used in our experiments was isolated in 1977 by J.S. Horoszewicz, et al. An adsorptive transfer stripping technique coupled with differential pulsed voltammetry Brdicka reaction was employed for the determination of metallothionein MT and AMACR level in cell lines extract. MT content was hundred of ug per g of soluble proteins and was higher as well as for AMACR compared to non-tumour cell line. |
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