In vitro activation of cytotoxic T-lymphocytes by hTERT-pulsed dendritic cells

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Authors

KRYUKOV Fedor OČADLÍKOVÁ Darina KOVÁŘOVÁ Lucie BUREŠOVÁ Ivana HÁJEK Roman MICHÁLEK Jaroslav

Year of publication 2009
Type Article in Periodical
Magazine / Source Journal of immunotoxicology
MU Faculty or unit

Faculty of Medicine

Citation
Field Oncology and hematology
Keywords multiple myeloma; T-lymphocyte; hTERT
Description In the studies reported here, we studied antigen-specific and HLA-A2-restricted cytotoxic activity against an ARH77 myeloma cell line in vitro. An HLA-A2-specific hTERT-derived nonapeptide ((540)ILAKFLHWL(548)) was used as a TAA. Myeloma-specific cytotoxic activity of hTERT-reactive cytotoxic lymphocytes (CTLs) was established by repeated stimulation of the CTLs via dendritic cells loaded with hTERT-derived nonapeptide. These studies were able to demonstrate that hTERT-reactive T-lymphocytes can be identified and expanded using relatively simple in vitro techniques consisting of antigen-specific stimulation, immunomagnetic sorting, and then induction of rapid expansion.
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