Mapping of powdery mildew resistance genes in a newly determined accession of Hordeum vulgare ssp. spontaneum
| Authors | |
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| Year of publication | 2010 |
| Type | Article in Periodical |
| Magazine / Source | Annals of Applied Biology |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | barley; Blumeria graminis f.sp. hordei; DNA markers; genetic mapping; Hordeum vulgare ssp. spontaneum; Mla; resistance genes |
| Description | The accession PI466197 of wild barley (Hordeum vulgare ssp. spontaneum) with a newly identified resistance to powdery mildew caused by Blumeria graminis f.sp. hordei was studied with the aim to localize the genes determining resistance on a barley genetic map using DNA markers. Molecular analysis was performed in the F2 population of the cross between the winter variety Tiffany and the resistant accession PI466197, consisting of 113 plants. One of the resistance genes detected was localized between the markers RGH1aE1 and Bmac0213 on the short arm of chromosome 1H, which is the position consistent with the known Mla locus. The other gene was proven to be highly significantly linked with the GBMS247, Bmac0134 and MWG878 markers on the short arm of chromosome 2H. The flanking markers were Bmac0134 and MWG878, assigned 4 and 8 cM from the gene, respectively. Until now, no gene conferring powdery mildew resistance originating from H. vulgare has been located on the short arm of barley chromosome 2H. |
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