RETENTION BEHAVIOUR OF OLIGONUCLEOTIDES ISOMERS STUDIED BY MEANS OF HYDROPHILIC INTERACTION CHROMATOGRAPHY

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Authors	FUKSOVÁ Hana HÉGROVÁ Blanka KOSTEČKA Pavel TRNKOVÁ Libuše BITTOVÁ Miroslava
Year of publication	2010
Type	Article in Proceedings
Conference	X. pracovní setkání fyzikálních chemiků a elektrochemiků - sborník příspěvků
MU Faculty or unit	Faculty of Science
Citation
Field	Analytic chemistry
Keywords	oligonucleotides; chromatography
Description	Short oligonucleotides and its isomers are deeply studied for better understanding of nucleic acid properties and interactions. High performance liquid chromatography (HPLC) provides suitable tool for their characterization. Our interest in this study was focused on the separation of pentamers, consisted of adenine, guanine and thymine by means of hydrophilic liquid chromatography mode (HILIC). The influence of experimental parameters such as mobile phase pH and/or buffer concentration, percentage of organic solvent in mobile phase and flow rate was evaluated and the most acceptable conditions were selected for further analysis. Finally, obtained results were compared with commonly applied reverse phase liquid chromatography (RP HPLC).
Related projects:	Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine