SPECTROSCOPIC STUDY OF PROTONATION EQULIBRIA OF SHORT OLIGONUCLEOTIDES CONTAINING CYTOSIN

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Authors	HOLUBOVÁ Sylvie LUBAL Přemysl TRNKOVÁ Libuše
Year of publication	2010
Type	Article in Proceedings
Conference	X. pracovní setkání fyzikálních chemiků a elektrochemiků - sborník příspěvků
MU Faculty or unit	Faculty of Science
Citation
Field	Analytic chemistry
Keywords	oligonucleotides; cytosine; equilibria; spectroscopy
Description	This paper deals with the spectroscopic study of protonation of short deoxyoligonucleotides (ODNs) containing cytosine. The measurements were performed at constant temperature and ionic strength. The influence of the number of cytosine in the ODNs chain on protonation was studied. The pKa values were calculated using the software OPIUM. Constants pKa determined at several temperatures provided the possibility to calculate the thermodynamic parameters such as enthalpy and entropy.
Related projects:	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL) Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine